Discrimination Between Child and Adult Forms Using Radar Frequency Signature Analysis

In this thesis we develop a method to discriminate between adult and child radar signatures. In particular, we examine radar data measured from behind a wall, which introduces radar signal attenuation and multipath effects. To investigate the child/adult discrimination problem in a through-wall, multipath scenario, a previously developed free-space human scattering model was expanded to incorporate multiple paths, and the effects of transmission through, and reflections from, walls and ground. The ground was modeled as a perfectly reflecting surface, while the walls were modeled as homogeneous concrete slabs. Twenty-five reflection paths were identified, involving the direct paths, as well as reflected paths between the ground and an adjacent wall. All paths included two-way transmission through an obstructing wall

Targeting Antioxidant Enzyme Expression as a Therapeutic Strategy for Ischemic Stroke

During ischemic stroke, neurons and glia are subjected to damage during the acute and neuroinflammatory phases of injury. Production of reactive oxygen species (ROS) from calcium dysregulation in neural cells and the invasion of activated immune cells are responsible for stroke-induced neurodegeneration. Scientists have failed thus far to identify antioxidant-based drugs that can enhance neural cell survival and improve recovery after stroke. However, several groups have demonstrated success in protecting against stroke by increasing expression of antioxidant enzymes in neural cells. These enzymes, which include but are not limited to enzymes in the glutathione peroxidase, catalase, and superoxide dismutase families, degrade ROS that otherwise damage cellular components such as DNA, proteins, and lipids. Several groups have identified cellular therapies including neural stem cells and human umbilical cord blood cells, which exert neuroprotective and oligoprotective effects through the release of pro-survival factors that activate PI3K/Akt signaling to upregulation of antioxidant enzymes. Other studies demonstrate that treatment with soluble factors released by these cells yield similar changes in enzyme expression after stroke. Treatment with the cytokine leukemia inhibitory factor increases the expression of peroxiredoxin IV and metallothionein III in glia and boosts expression of superoxide dismutase 3 in neurons. Through cell-specific upregulation of these enzymes, LIF and other Akt-inducing factors have the potential to protect multiple cell types against damage from ROS during the early and late phases of ischemic damage

The Role of the Leukemia Inhibitory Factor Receptor in Neuroprotective Signaling

Several neurotropic cytokines relay their signaling through the leukemia inhibitory factor receptor. This 190kDa subunit couples with the 130kDa gp130 subunit to transduce intracellular signaling in neurons and oligodendrocytes that leads to expression of genes associated with neurosurvival. Moreover, activation of this receptor alters the phenotype of immune cells to an anti-inflammatory one. Although cytokines that activate the leukemia inhibitory factor receptor have been studied in the context of neurodegenerative disease, therapeutic targeting of the specific receptor subunit has been understudied in by comparison. This review examines the role of this receptor in the CNS and immune system, and its application in the treatment in stroke and other brain pathologies

Precipitation Drivers of Cropping Frequency in the Brazilian Cerrado: Evidence and Implications for Decision-Making

The Amazon basin has been subjected to unprecedented rates of land-use change over the past several decades, primarily as a result of the expansion of agriculture. Enhanced rain forest conservation efforts toward the end of the twentieth century slowed deforestation of the Amazon but, in turn, increased demand for land repurposing in the adjacent Cerrado (savanna) region, where conservation regulations are less strict. To maintain or increase yields while minimizing the need for additional land, agricultural producers adopted a form of intensification in which two rain-fed crops are planted within a single growing season (double cropping). Using 10 years (August 2002 to July 2012) of MODIS and TRMM data, it is demonstrated that there exists a threshold growing season rainfall amount (1759 mm) for double cropping. But more nuanced is the relationship between observable precipitation information available to farmers at the time of planting decision and the choice to “double crop” in a given year. An evaluation of decision-available precipitation characteristics provides strong evidence for the importance of high rainfall frequency during a critical period prior to, and including, the rainy season onset

Open For Business: Analyzing Venetian Storefronts

Like doctors diagnosing ailments, Venice’s shops act as indicators for underlying trends occurring throughout the city. Over the past 50 years, Venice’s population has seen a large increase in tourists and a sharp decline in lifelong residents. To draw meaningful conclusions about the trends caused by this demographic shift, data about business in Venice must be collected. The goal of this project is to collect data about the type, number, and location of stores in Venice, and to add this data to the VPC’s database. The website that displays this data will also be updated with a modern look. The data will then be visualized using the website and analyzed to show how demographic shifts may be correlated to changes in Venice’s retail landscape

The Poststroke Peripheral Immune Response Is Differentially Regulated by Leukemia Inhibitory Factor in Aged Male and Female Rodents

Background. The goal of this study was to determine whether leukemia inhibitory factor (LIF) promotes anti-inflammatory activity after stroke in a sex-dependent manner. Methods. Aged (18-month-old) Sprague-Dawley rats of both sexes underwent sham surgery or permanent middle cerebral artery occlusion (MCAO). Animals received three doses of intravenous LIF (125 μg/kg) or PBS at 6, 24, and 48 h before euthanization at 72 h. Spleen weights were measured immediately following euthanization. Western blot was used to measure protein levels of CCL8, CD11b, CXCL9, CXCL10, IL-12 p40, IL-3, and the LIF receptor (LIFR) in spleen tissue. ELISA was used to measure IL-1β, IL-6, TNFα, and IFNγ in spleen tissue. A Griess Assay was used to indirectly quantify NO levels via measurement of nitrite. Levels of cellular markers and inflammatory mediators were normalized to the baseline (sham) group from each sex. Statistical analysis was performed using two-way ANOVA and followed by Fisher’s LSD post hoc test. Results. Aged female rats showed a significantly lower spleen weight after MCAO, but showed a significant increase in spleen size after LIF treatment. This effect was observed in aged male rats, but not to as great of an extent. CD11b levels were significantly higher in the spleens of MCAO+PBS males compared to their female counterparts, but there was no significant difference in CD11b levels between MCAO+LIF males and females. LIF significantly increased CXCL9 after LIF treatment in aged male and female rats. LIFR and IL-3 were upregulated after LIF treatment in aged females. Splenic nitrate increased after MCAO but decreased after LIF treatment in aged females. Splenic nitrate levels did not increase after MCAO but did increase after LIF treatment in aged males. The following cytokines/chemokines were not altered by sex or treatment: TNFα, IL-6, IL-12 p40, CCL8, IFNγ, and CXCL10. Conclusions. LIF treatment after permanent MCAO induces sex-dependent effects on the poststroke splenic response and the production of proinflammatory cytokines among aged rats

The Relationship between Tropical Cyclone Activity, Nutrient Loading, and Algal Blooms over the Great Barrier Reef

The Great Barrier Reef, the world’s largest coral reef ecosystem, is subject to many environmental stressors. This study utilizes remotely sensed Moderate Resolution Imaging Spectroradiometer (MODIS) chlorophyll a concentration data to explore statistically significant relationships between local-scale tropical cyclone disturbance and relative water quality between 2004–2014. The study reveals that tropical cyclone activity reduces water quality at 8- and 16-day time lags. Relationships suggest that at early stages (during and just after cyclone activity) algal response is induced primarily through wind-driven sediment re-suspension. However, wind speed in isolation only increases minimum levels of chlorophyll a, rather than mean or extreme upper values. At greater time lags (16-day), it is suggested that nutrient runoff from rainfall (and perhaps storm surge) increase phytoplankton activity, leading to detrimental ecological effects. The analyses systematically demonstrate the dominance of tropical cyclone size on mean and extreme values of chlorophyll a during and after tropical cyclone activity (at 0-, 8-, and 16-day time lags). Both the total area affected and the area from which nutrients can be extracted have more impact on chlorophyll a concentrations than either the duration or intensity of the cyclone. Findings indicate that efforts to reduce nutrient and sediment leaching into the reef lagoon from the Queensland coastal lands need to be continued and improved. This will be particularly important in the context of climate change, since tropical cyclone frequency, dynamics and characteristics are likely to change

Spillover adherence effects of fixed-dose combination HIV therapy

The impact of fixed-dose combination (FDC) products on adherence to other, non-fixed regimen components has not been examined. We compared adherence to a third antiretroviral (ART) component among patients receiving a nucleoside reverse transcriptase inhibitor (NRTI) backbone consisting of the FDC Epzicom®, GlaxoSmithKline Inc, Research Triangle Park, NC (abacavir sulfate 600 mg + lamivudine 300 mg; FDC group) versus NRTI combinations taken as two separate pills (NRTI Combo group) using data from a national sample of 30 health plans covering approximately 38 million lives from 1997 to 2005. Adherence was measured as the medication possession ratio (MPR). Multivariate logistic regression compared treatment groups based on the likelihood of achieving ≥95% adherence, with sensitivity analyses using alternative thresholds. MPR was assessed as a continuous variable using multivariate linear regression. Covariates included age, gender, insurance payer type, year of study drug initiation, presence of mental health and substance abuse disorders, and third agent class. The study sample consisted of 650 FDC and 1947 NRTI Combo patients. Unadjusted mean adherence to the third agent was higher in the FDC group than the NRTI Combo group (0.92 vs 0.85; P < 0.0001). In regression analyses, FDC patients were 48% and 39% more likely to achieve 95% and 90% third agent adherence, respectively (P ≤ 0.03). None of the other MPR specifications achieved comparable results. Among managed care patients, use of an FDC appears to substantially improve adherence to a third regimen component and thus the likelihood of achieving the accepted standard for adherence to HIV therapy of 95%

NK cell education: Physiological and pathological influences

Natural killer (NK) cells represent a critical defense against viral infections and cancers. NK cells require integration of activating and inhibitory NK cell receptors to detect target cells and the balance of these NK cell inputs defines the global NK cell response. The sensitivity of the response is largely defined by interactions between self-major histocompatibility complex class I (MHC-I) molecules and specific inhibitory NK cell receptors, so-called NK cell education. Thus, NK cell education is a crucial process to generate tuned effector NK cell responses in different diseases. In this review, we discuss the relationship between NK cell education and physiologic factors (type of self-MHC-I, self-MHC-I allelic variants, variant of the self-MHC-I-binding peptides, cytokine effects and inhibitory KIR expression) underlying NK cell education profiles (effector function or metabolism). Additionally, we describe the broad-spectrum of effector educated NK cell functions on different pathologies (such as HIV-1, CMV and tumors, among others)

Expression of BK-type calcium-activated potassium channel splice variants during chick cochlear development

The appearance of large-conductance, calcium-activated potassium (BK) current is a hallmark of functional maturation in auditory hair cells. Acquisition of this fast-activating current enables high-frequency, graded receptor potentials in all vertebrates and an electrical tuning mechanism in nonmammals. The gene encoding BK Α subunits is highly alternatively spliced, and the resulting variations in channel isoforms may contribute to functional diversity at the onset of hearing. We examined the tissue specificity of nine BK Α alternative exons and investigated changes in expression during chick cochlear development using quantitative polymerase chain reaction (qPCR). Each alternative was widely expressed in several tissues except for an insert near the C-terminus Ca 2+ sensing domain, which appeared brain-specific. The only alternative form in the membrane-bound core of the channel was expressed in brain and muscle but was undetected in cochlea. Of the remaining variants, three increased in expression prior to the onset of hearing and acquisition of BK currents. These three variants cause decreased Ca 2+ sensitivity or increased intracellular retention, traits that would not easily explain the advent of calcium-sensitive currents at embryonic day (E)18–19. Expression levels of other variants were mature and stable by E15, days before currents were acquired. Surface expression of C-terminal isoforms was examined using patch-clamp electrophysiology and immunocytochemistry. C-terminal variants that exhibit robust surface expression appeared in the membrane at E18, even though transcripts were unchanged during development starting from E12. These results indicate that delays in protein synthesis and trafficking/scaffolding of channel subunits underlie the late acquisition of BK currents in cochlear hair cells. J. Comp. Neurol. 518:2554–2569, 2010. © 2010 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75768/1/22352_ftp.pd